Prevalence and extent of heteroresistance by next generation sequencing of multidrug-resistant tuberculosis
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چکیده
منابع مشابه
Correction: Prevalence and extent of heteroresistance by next generation sequencing of multidrug-resistant tuberculosis
[This corrects the article DOI: 10.1371/journal.pone.0176522.].
متن کاملPrevalence and extent of heteroresistance by next generation sequencing of multidrug-resistant tuberculosis
Amplicon-based Next Generation Sequencing (NGS) is an emerging method for Mycobacterium tuberculosis drug susceptibility testing (DST) but has not been well described. We examined 158 clinical multidrug-resistant M. tuberculosis isolates via NGS of 11 resistance-associated gene regions covering 3519 nucleotides. Across these gene regions, complete resistance or heteroresistance (defined as 1%-9...
متن کاملStrategies and Clinical Applications of Next Generation Sequencing
Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...
متن کاملStrategies and Clinical Applications of Next Generation Sequencing
Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...
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Technical Appendix Figure. Whole-genome, short-sequencing reads of Mycobacterium tuberculosis strain 4542 mapped to complete genome of reference strain H37Rv (NC_00962.3) by using Geneious 9.0 package: genome locus of spacers 7–9 in DR/CRISPR locus. Spacer numbers are given according to 43-spoligotyping (1). DR/CRISPR locus in M. tuberculosis is located on complementary strand, whereas spacers ...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2017
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0176522